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. 2016 Sep 23;22:3383–3393. doi: 10.12659/MSM.897301

Figure 5.

Figure 5

Insulin inhibits miR-483 and promotes IGF1 and KGN cell proliferation. (A) The miR-483 levels detected by qRT-PCR in cells treated with 0, 1, 10, and 100 ng/mL insulin for 48 h. (B) The IGF1 mRNA levels detected by qRT-PCR in cells treated with 0, 1, 10, and 100 ng/mL insulin for 48 h. (C) Insulin (100 ng/mL) promotes KGN cell viability compared to the untreated cells (control). Cell viability was detected with MTT method after 24, 48, or 72 h of insulin treatment. (D) Pictures of colony formation assay. (E) Colony formation rate was increased by insulin (100 ng/mL) detected by colony formation assay after 48 h of insulin treatment. * P<0.05. ** P<0.01. *** P<0.001. IGF1, insulin-like growth factor 1.