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. 2016 Sep 28;10(9):e0005013. doi: 10.1371/journal.pntd.0005013

Fig 2. ATP-dependent DNA supercoiling assay.

Fig 2

Supercoiling activities of DNA gyrases composed of WT-GyrA, Gly89Cys-GyrA, Ala91Val-GyrA or Asp95Gly-GyrA and WT-GyrB were confirmed. Relaxed pBR322 DNA was incubated with GyrA, GyrB, or both, of the subunits in the presence or absence of ATP. Lane 1: relaxed pBR322 DNA alone; lanes 2–6: relaxed pBR322 DNA, ATP, GyrA, and GyrB; lanes 7–11: relaxed pBR322 DNA, ATP, and GyrA; lane 12: relaxed pBR322 DNA, ATP, and GyrB; lane 13–17: relaxed pBR322 DNA, GyrA, and GyrB. * Twice the amount of DNA gyrase subunits was used in these assays.