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Supercoiled pBR322 DNA was mixed and incubated with GyrA, GyrB, ATP and FQs at the indicated concentrations. Each FQ was examined for its cleaving activity against WT DNA gyrase and mutant DNA gyrases with Gly89Cys-GyrA (indicated as Gly89Cys), Ala91Val-GyrA (Ala91Val) and Asp95Gly-GyrA (Asp95Gly). The lanes labeled as SC and L indicate supercoiled and linearized pBR322 DNA, respectively.
