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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Pharm Biol. 2016 Jan 20;54(9):1503–1512. doi: 10.3109/13880209.2015.1107104

Figure 2.

Figure 2

Activation of Nrf2 and HSF1. A. Luciferase reporter assay of Nrf2 and HSF1 following FKA or FKB treatment. HepG2 were transfected with firefly luciferase reporter constructs for Nrf2 (pGL3-ARE), HSF1 (pGL4-HSE), or their respective parent vectors (pGL3-Promoter, pGL4.27) as empty plasmid controls. Transfected cells were subsequently treated with FKA, FKB, or vehicle control (0.1% DMSO) for 24 h and luciferase was measured. EC50 values for the enhancement of luciferase expression were calculated by nonlinear regression as described in Materials and methods. B. Nuclear expression of Nrf2 and HSF1 is enhanced by FKA or FKB. HepG2 were treated with the indicated concentrations of FKA, FKB, or vehicle control (0.1% DMSO, 0 μM) for 24 h and total protein was collected and analyzed by Western blot using nuclear β-actin levels as a loading control.