Fig 4. DAVID function analysis of identified genes.

A. DAVID functional analysis was performed to examine potential functional association of identified genes. Genes in the four major modules (black and purple modules from NPC model and blue and brown modules from neural rosette model) were analyzed. It was found that genes dysregulated upon EtOH treatment in both NPCs and neural rosettes are significantly associated with neuroactive ligand-receptor interaction, JAK-STAT pathway and cytokine-cytokine receptor interaction and TLR signaling pathway. B. Genes that are commonly affected by EtOH (greater than 3-fold) in neural rosette and NPC were identified by Venny plot. List of genes are shown in Table 1.