Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Sep 28;11(9):e0163812. doi: 10.1371/journal.pone.0163812

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Kim et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PMC Copyright notice

Fig 6 — A. Comparison of fold changes of P2RX3 expression in undifferentiated H1 hESCs and hESC-derived NPCs treated with EtOH. P2RX3 in undifferentiated hESCs was downregulated and conversely upregulated in NPCs with increased EtOH concentration. Bars are mean ± SEM from triplicates; significant (p<0.05) difference from control (one-way ANOVA). B. The level of CNTNAP4 and P2RX3 in H1 NPC was induced by treatment with 20 mM EtOH. Co-treatment with 1 μM dihydromyricetin (DHM), a flavonoid component of herbal medicines that counters acute EtOH intoxication, significantly interfered the effect of EtOH, demonstrating the specificity of EtOH’s effect on the gene expression. Bars are mean ± SEM from triplicates; significant (p<0.05) difference from control (one-way ANOVA). C. Relative protein levels of P2RX3 in undifferentiatied H9 hESCs and H9 NPC cell lines treated with 0, 20, and 50 mM EtOH for 48 hrs. From Western analysis, we have confirmed alcohol-induced downregulation of P2RX3 in undifferentiated hESCs and upregulation in NPCs.