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. Author manuscript; available in PMC: 2017 Sep 27.
Published in final edited form as: Circulation. 2016 Aug 31;134(13):961–977. doi: 10.1161/CIRCULATIONAHA.116.021618

FIGURE 5. In vivo CICR by AAV9-GCaMP6f fluorescence.

FIGURE 5

A. Example fluorescence measurements of CICR (y-axis F/F vs x-axis time). In vivo fluorescence imaging was performed 3 or 4 weeks after GCaMP6f delivery in rat (3w P.D., 4w P.D.). B. Example image of raw fluorescence seen through fiber optic on heart by camera at baseline and peak fluorescence during a calcium transient. C. Example cardiac arrest experiment from a different GECI-rat while measuring CICR fluorescence continuously through experiment (y-axis F/F vs x-axis time). Shown were measurements at baseline, after cardiac arrest, on ECMO demonstrating delayed calcium release initiating fibrillatory behavior similar to what was seen in isolated cells, and finally off ECMO.