Table 1.
Nineteen amino acid positions identified as significantly varying between NS1 sequences from all 4 DENV serotypes.
| NS1 Position12 |
Polyprotein Position |
P-value | Type 1 Consensus Residue (%) |
Type 2 Consensus Residue (%) |
Type 3 Consensus Residue (%) |
Type 4 Consensus Residue (%) |
|---|---|---|---|---|---|---|
| 47 | 823 | <1.0E-340 | G (100) | Q (99) | A (100) | L (98) |
| 83 | 859 | <1.0E-340 | D (97) | E (100) | N (99) | G (100) |
| 84 | 860 | <1.0E-340 | M (97) | V (97) | I (99) | H (100) |
| 98 | 874 | <1.0E-340 | A (91) | Q (99) | E (99) | T (77) |
| 103 | 879 | <1.0E-340 | M (97) | S (98) | T (98( | A (95) |
| 124 | 900 | <1.0E-340 | I (100) | L (98) | V (99) | F (100) |
| 128 | 904 | <1.0E-340 | V (66) | S (77) | T (89) | A (83) |
| 139 | 915 | 7.07E-193 | N (70) | E (99) | N (97) | D (100) |
| 146 | 922 | <1.0E-340 | D (81) | T (94) | A (90) | E (100) |
| 147 | 923 | <1.0E-340 | Q (100) | N (100) | S (97) | R(100) |
| 152 | 928 | <1.0E-340 | I (100) | S (100) | V (100) | F (50) |
| 174 | 950 | <1.0E-340 | S (100) | K (87) | V (83) | G (100) |
| 191 | 967 | <1.0E-340 | S (99) | N (95) | E (100) | Q (100) |
| 205 | 981 | <1.0E-340 | E (100) | A (94) | Q (100) | S (100) |
| 208 | 984 | <1.0E-340 | E (100) | D (100) | G (100) | Q (100) |
| 224 | 1000 | <1.0E-340 | I (79) | H (96) | T (99) | L (100) |
| 246 | 1022 | 1.62E-280 | I (95) | N (92) | S (99) | S (75) |
| 279 | 1055 | <1.0E-340 | L (99) | F (91) | Y (100) | E (100) |
| 324 | 1100 | 4.90E-324 | R (53) | R (99) | M (98) | L (100) |
1
NS1 amino acid position 1 corresponds to polyprotein amino acid position 776 of the DENV-2 S1 vaccine strain.
2
Significant positions that overlap with experimentally-determined immune epitopes from the IEDB database are highlighted in bold.