Table 1.
Impact of pressure on rate constants and KIEs at the extremes of the experimental temperature range. [a]
| SLO | T (K) | 344 bar | 688 bar | 1034 bar | |||
|---|---|---|---|---|---|---|---|
|
| |||||||
| S (kcat-H) | S (kcat-D) | S (kcat-H) | S (kcat-D) | S (kcat-H) | S (kcat-D) | ||
| WT | 288 | 1.00 (0.05) | 0.98 (0.03 | 0.97 (0.08) | 1.17 (0.02) | 0.97 (0.09) | 1.22 (0.03) |
| 308 | 1.40 (0.18) | 1.32 (0.06) | 1.50 (0.10) | 1.69 (0.07) | 1.77 (0.08) | 2.10 (0.11) | |
|
| |||||||
| I553V | 288 | 1.08 (0.09) | 1.02 (0.02) | 1.10 (0.13) | 1.07 (0.01) | 1.01 (0.19) | 1.25 (0.02) |
| 308 | 1.10 (0.02) | 1.00 (0.02) | 1.43 (0.03) | 1.27 (0.02) | 1.66 (0.03) | 1.82 (0.04) | |
|
| |||||||
| L546A | 288 | 1.03 (0.02) | 1.14 (0.02) | 1.20 (0.06) | 1.32 (0.04) | 1.28 (0.03) | 1.56 (0.03) |
| 308 | 1.07 (0.02) | 1.28 (0.04) | 1.42 (0.07) | 1.55 (0.04) | 1.68 (0.07) | 1.95 (0.03) | |
|
| |||||||
| L754A | 208 | 1.58 (0.10) | N.D.[b] | 2.92 (0.14) | N.D.[b] | 4.24 (0.24) | N.D.[b] |
| 308 | 1.90 (0.13) | N.D.[b] | 3.35 (0.20) | N.D.[b] | 5.26 (0.44) | N.D.[b] | |
[a]
S is the ratio of kinetic parameters at the pressure listed relative to ambient pressure (Table S3–S6).
[b]
The kcat-D values were too slow to be accurately determined.