Mitochondrial respiration of permeabilized skeletal muscle fibers from gluteus medius and triceps brachii. Mass-specific O2 flux (pmol O2·s−1·mg wet wt−1; A–D) and O2 flux normalized to CS activity (pmol·s−1·unit CS−1; E–H), respectively, with LEAK respiration (A and E), OXPHOS capacityCI (B and F), OXPHOS capacityCI+II (C and G), and maximal ETS capacity (D and H) are shown. Values are means ± SE; n = 17–18 (young-gluteus), 12 (young-triceps), 9 (aged-gluteus), and 9 (aged-triceps). Open bars represent young horses; solid bars, aged horses. Young vs. aged: †P < 0.1, *P < 0.05. Gluteus vs. triceps: †P < 0.1, §P < 0.05, §§§P < 0.001.