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. 2016 Aug 20;5:e17877. doi: 10.7554/eLife.17877

Figure 2. Exosome complex disruption renders primary erythroid cells hypersensitive to limiting erythropoietin concentrations.

Figure 2.

(A) Flow cytometric analysis with Annexin V and the membrane-impermeable dye DRAQ7 to quantitate apoptosis with control and Exosc8-knockdown primary erythroid cells expanded for 48 hr under Epo-limiting conditions. (B) Quantification of the percentage of primary erythroid cells in live, late and early apoptotic populations (mean ± SE, 4 biological replicates). Statistical analysis of control and treatment conditions was conducted with the Student’s T-test. *p<0.05, **p<0.01, ***p<0.001. Source data is available in Figure 2—source data 1.

DOI: http://dx.doi.org/10.7554/eLife.17877.006

Figure 2—source data 1. This Excel spreadsheet contains the values of each biological replicate for data presented as line graphs (mean ± SE) in Figure 2.
Sheet 1: Figure 2B percentage of cells from each biological replicate found in the live, early apoptotic, late apoptotic and necrotic flow cytometry gates in control and Exosc8 knockdown cells after 48 hr culture under Epo-limiting conditions.
DOI: http://dx.doi.org/10.7554/eLife.17877.007
elife-17877-fig2-data1.xlsx (18.6KB, xlsx)
DOI: 10.7554/eLife.17877.007