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. 2016 Aug 20;5:e17877. doi: 10.7554/eLife.17877

Figure 6. Exosome complex occupies the Kit locus and is required for active RNA Polymerase II occupancy at Kit.

Figure 6.

(A) qChIP of serine 5-phospho Pol II, and GATA-1 occupancy at Kit in control and Exosc8-knockdown Ter119- erythroid precursor cells 24 hr post-infection (mean ± SE, 6 independent experiments). (B) qChIP of Exosc9 occupancy at Kit and promoters of other exosome complex-regulated erythroid genes (Alas2, Hbb-b1 and Slc4a1) in erythroid precursor cells after culturing for 48 hr (mean ± SE, 3 biological replicates). Statistical analysis of control and treatment conditions was conducted with the Student’s T-test. *p<0.05, **p<0.01, ***p<0.001. Source data is available in Figure 6—source data 1.

DOI: http://dx.doi.org/10.7554/eLife.17877.016

Figure 6—source data 1. This Excel spreadsheet contains the values for each biological replicate for data presented in histograms (mean ± SE) in Figure 6.
Sheet 1: Figure 6A qChIP of serine 5-phospho Pol II, and GATA-1 occupancy at Kit in control and Exosc8-knockdown Ter119- erythroid precursor cells 24 hr post-infection. Sheet 2: Figure 6B qChIP of Exosc9 occupancy at Kit and promoters of other exosome complex-regulated erythroid genes (Alas2, Hbb-b1 and Slc4a1) in erythroid precursor cells after culturing for 48 hr.
DOI: http://dx.doi.org/10.7554/eLife.17877.017
elife-17877-fig6-data1.xlsx (57KB, xlsx)
DOI: 10.7554/eLife.17877.017