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. 2016 Aug 20;5:e17877. doi: 10.7554/eLife.17877

Figure 7. Functional link between Kit downregulation and erythroid differentiation induced by disrupting the exosome complex.

Figure 7.

(A) Erythroid maturation analyzed by flow cytometric quantitation of CD71 and Ter119 post-Exosc8 knockdown and/or Kit expression in primary erythroid precursor cells expanded for 72 hr. Representative flow cytometry plots, with the R1-R5 gates denoted. (B) Left: relative Kit MFI post-Exosc8 knockdown and/or Kit overexpression (mean ± SE, 4 biological replicates). Right: percentage of primary erythroid precursor cells in the R1-R4 gates (mean ± SE, 4 biological replicates). (C) Left: relative Kit MFI 48 hr post-Exosc8 knockdown in cells infected with increasing amounts of a Kit-expressing retrovirus. The arrow depicts Kit downregulation resulting from knocking-down Exosc8. Right: percentage of erythroid precursor cells in the R3 population 48 hr post-infection with shExosc8 in cells infected with increasing amounts of Kit-expressing retrovirus. The arrow depicts the increased R3 population post-Exosc8 knockdown. ANOVA identified any significant variation between experimental groups then a Tukey-Kramer test identified the statistical relationship between each pair of samples, *p<0.05, **p<0.01, ***p<0.001. Source data is available in Figure 7—source data 1

DOI: http://dx.doi.org/10.7554/eLife.17877.018

Figure 7—source data 1. This Excel spreadsheet contains the values for each biological replicate for data presented in line graphs (mean ± SE) in Figure 7.
Sheet 1 2 and 3: Figure 7B Kit MFI and percentage of erythroid precursor cells in the R1, R2, R3 R4 and R5 populations 24, 48 and 72 hr post Exosc8 knockdown and/or Kit overexpression. Sheet 4: Figure 7C Kit MFI and percentage of erythroid precursor cells in the R1, R2, R3, R4 and R5 population 48 hr post-Exosc8 knockdown in cells infected with increasing amounts of a Kit-expressing retrovirus.
DOI: http://dx.doi.org/10.7554/eLife.17877.019
elife-17877-fig7-data1.xlsx (27.5KB, xlsx)
DOI: 10.7554/eLife.17877.019