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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: J Appl Toxicol. 2016 Mar 28;36(11):1446–1459. doi: 10.1002/jat.3300

Figure 4.

Figure 4

Arsenic does not inhibit thapsigargin (Tg) -induced degranulation. (A) Relative degranulation response of RBL-2H3 cells treated for 1hr with 4.6 nM Tg (which caused an average absolute degranulation response of 11.9 + 3.9% [SEM]), ± As. Values represent mean ± SEM of 3-5 experiments of triplicate samples per dose per experiment. A spontaneous release measurement (no Tg or As present) is depicted for reference. No significant difference was determined by one-way ANOVA. In (B) trypan blue exclusion assays, the percentage of living cells present in the 0 nM Tg control is plotted against concentration of Tg (1.5 and 4.6 nM); all samples contain 750 ppb As. Values are means of two experiments, each with triplicate samples, where data were normalized to the 0 control (with no Tg and no As, but containing Tg vehicle of 0.001% DMSO). No significant difference was determined by one-way ANOVA. (C) Short-term cytotoxicity was determined using an LDH cytotoxicity detection kit; concentration of Tg is plotted against the percentage LDH released in the absence or presence of 750 ppb As; values represent individual wells (n = 6-9), and error bars are SD. No significant difference was determined by one-way ANOVA.