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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: J Appl Toxicol. 2016 Mar 28;36(11):1446–1459. doi: 10.1002/jat.3300

Figure 5.

Figure 5

Arsenic does not affect c48/80-induced degranulation of RBL-2H3 cells. (A) Effect of As on c48/80-mediated degranulation. Cells were pre-treated with quercetin and then co-exposed to 25 μg mL-1 c48/80 (which caused an average absolute degranulation response of 7.7% ± 0.6% [SEM]) and varying concentrations of As (0-750 ppb) for 15 minutes, followed by β-hexosaminidase quantification as described in “Methods.” Data were normalized to the 0 ppb As response value of each experiment (n = 5). (B) LDH cytotoxicity assay indicating non-cytotoxicity of 25 μg mL-1 c48/80 treatment (n = 3). Cells were treated as in (A), and LDH release was measured as described in “Methods.” In (A-B), values represent means ± SEM; statistical significance was determined by one-way ANOVA followed by Tukey's post test (in A, comparison made to 100 ppb; in B, direct comparison is made to 0 μg mL-1 c48/80); *p< 0.05, ***p< 0.001.