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The karyotype and FISH analysis of ALL3 cells. A and B. 26.5% of ALL3 cells have 2 copies of bcr/abl and 73.5% have 3 copies of bcr/abl. C. Confirmation of size of BCR/ABL protein in ALL3 cells using western blot analysis. The ALL3 have a p190 kd size fusion protein as compared with other cell lines such as K562, R10(-) cell lines and CML CD34+ cells which contain a p210 kd BCR/ABL protein. Note: Analysis was performed by Cytogenetics core facility, MSKCC, NY.
