Figure 5.

Growth of the ALL3 cells at different starting cell densities per ml and per cm² of surface area. A. Comparison of the growth of ALL3 cells at different starting densities in 96-well round bottom plates. The y-axis represents total number of viable cells per ml. Note: Because the well volume is only 0.2 ml and the bottom surface area only 0.32 cm², the total cell numbers per well are 1/5 of those shown per ml and 3 times the latter per cm². The doubling times shown are very rough estimates because of low cell counts. B. The [³H]-Thymidine uptake assays showing comparison of ALL3 cells kept at different starting cell densities in 96-well round bottom plates. The y-axis represents the counts per minute (CPM). C. Comparison of growth of ALL3 cells at starting cell densities of 5000, 10000, and 20000 cells per ml and per cm² of surface area, in different size flasks, petri dishes or wells. D. Growth of ALL3 cells at different cell densities per ml and per cm² of bottom growing area in 24 well plates. E-G. Comparison of growth of ALL3 cells at starting cell densities of 5000, 10000, and 20000 cells/ml and per cm² of surface area in different size flasks, petri dish or wells.