Figure 1.

A-C: Paraffin sections were prepared and stained with anti-OPN pAb (green) and DAPI (blue) to stain DNA. OPN expression was inhibited by OPN-shRNA in OPN-shRNA group. Percentage of green stained area: sham group 2.96%±1.02%, vehicle group 3.19%±1.59%, OPN-shRNA group 0.21%±0.14%, n=3, scan bar, 25 μm, *P<0.001. D-F: Interstitial fibrosis of heart assessed by Masson staining, fibrotic area is stained blue, scan bar, 200 μm. Percentage of fibrosis area: sham group 16.72%±3.24%, vehicle group 15.82%±1.80%, OPN-shRNA group 5.86%±1.59%, n=5, *P<0.001.