Figure 2.

DNA methylation level of miR-145 promoter was increased in BMP2-inducing NSCs. NSCs were treated with 0 or 20 ng/ml of BMP2 for two weeks. A. Sequencing of bisulfite-treated DNA encoding miR-145 in cells and the methylation level of miR-145 promoter in the NSCs. B. The binding of miR-145 promoter with DNMT1, DNMT3a, or DNMT3b were examined by ChIP. Each line represents a single clone. Black boxes, methylated CpG sites; white boxes, unmethylated CpG sites; **VS control NSCs, P<0.01; n=6.