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. 2016 Sep 15;8(9):3710–3730.

Figure 4.

Figure 4

BMP9 induces the expression of early downstream genes and multiple lineage regulators in iMADs. A. Upregulation of early target genes in iMADs by BMP9. Subconfluent iMADs were infected with Ad-BMP9 or Ad-GFP. Total RNA was isolated at 36 h and subjected to TqPCR analysis using gene-specific primers for mouse Smad7, Id1, Ctgf and Hey1. B. BMP9 induces the expression of multiple lineage regulators/markers. Subconfluent iMADs were infected with Ad-BMP9 or Ad-GFP. Total RNA was isolated at the indicated time points and subjected to TqPCR analysis using gene-specific primers for Runx2, Osx, Ocn, Sox9 (day 7), or Pparγ2 (day 7). Gapdh was used as a reference gene. Reactions were done in triplicate. “*”, p<0.05, “**”, p<0.01.