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. 2016 Sep 15;8(9):3802–3811.

Figure 4.

Figure 4

miR-561 downregulates c-Myc through interaction with its 3’UTR. A. The sequences of miR-561 binding sites within the human c-Myc 3’UTRs and schematic reporter constructs, in this panel, c-Myc-WT represent the reporter constructs containing the entire 3’UTR sequences of c-Myc. C-Myc-MUT represent the reporter constructs containing mutated nucleotides. B. The analysis of the relative luciferase activities of c-Myc-WT, c-Myc-MUT. The error bars are derived from triplicate experiments. C. qRT-PCR analysis of c-Myc mRNA expression in HGC-27 cells after treatment with miRNA mimics or scramble or no transfection. The expression of c-Myc was normalized to GAPDH. D. Western blot analysis of c-Myc expression in HGC-27 cells transfected with miR-561 mimics or scramble or no transfection. GAPDH was also detected as a loading control. E. The relative expression of c-Myc was shown.