Figure 5.

Overexpression of c-Myc partially rescued miR-561-inhibited cell proliferation and invasion. A. Western blot analysis of c-Myc in HGC-27 cells co-transfected with either miR-561 mimic or scramble and 2.0 μg pCDNA-c-Myc or pCDNA empty vector. GAPDH was also detected as a loading control. B. Cell growth curves in HGC-27 cells transfected with different combinations at 0, 24, 48 and 72 h. C. Transwell analysis of HGC-27 cells treated with different combinations. The relative ratio of invasive cells per field is shown right. *p<0.05, **p<0.01, ***p<0.001.