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. 2016 Sep 15;8(9):3848–3860.

Figure 2.

Figure 2

TWEAK inhibited the apoptosis of H9C2 cells under hypoxia (H)/re-oxygenation (N). A: The apoptosis of H9C2 cells was induced by hypoxia for 24 h/reoxygenation for different durations (AnnexinV and PI staining; flow cytometry). H 24 h/N 24 h was employed to treat H9C2 cells, and the apoptosis rate was 71.47%. B: H9C2 cells were treated with rhTWEAK at different concentrations and exposed to H 24 h/N 24 h. TWEAK inhibited the apoptosis of H9C2 cells significantly in a dose-dependent manner when the rhTWEAK concentration was >10 ng/ml. C: The caspase-3 activation was inhibited significantly by rhTWEAK when the rhTWEAK concentration was >50 ng/ml. (*P<0.05, **P<0.01, ***P<0.001 vs. rhTWEWAK 0 ng/ml).