Figure 1.

Percentages of Th22, Th17, and Th1 cells and Th22 chemokine receptors expressed in both blood and kidney. A. Th22, Th17, and Th1 cells within CD4⁺ T cells were identified based on their expression of CD3⁺ and CD4⁺. B. Representative flow chart of Th22, Th17, and Th1 cells in the blood. C. Representative flow chart of CCR4⁺, CCR6⁺, and CCR10⁺ within IL-22⁺ T cells in the blood. D. Percentages of Th22 cells and their cell surface receptors CCR4, CCR6, and CCR10 presented increased values in IgAN mice and even higher values in HS-IgAN but were significantly downregulated in the CCL intervention group and the combination group (n=3 per group). Horizontal bars indicate the mean ± SE. The percentages of Th cells and CCRs were determined by flow cytometry, and appropriate species-matched Abs served as isotype controls.