Figure 3.

Chemokines in the kidney were chemotactic for IL-22-producing Th22 cells. CCL20, CCL22, and CCL27 were chemotactic for Th22 cells in vitro. Kidney tissue homogenates were used to stimulate the chemotaxis of Th22 cells in the absence or presence of anti-CCL20, anti-CCL22, and/or anti-CCL27 mAbs or a normal control. The chemotaxis index was calculated by dividing Th22 cell numbers that migrated in response to kidney homogenates by Th22 cell numbers that migrated in response to medium alone. Comparisons were determined by performing Kruskal-Wallis one-way analysis of variance for ranking. *P<0.05 compared with control.