Figure 3.

Panobinostat synergizes with ABT-199 in primary patient samples determined by MTT assays and standard isobologram analyses. Primary AML patient samples were treated with ABT-199 and panobinostat, alone or in combination, for 72 h and then viable cells were determined using MTT reagent. The IC₅₀ values are means of duplicates from one experiment due to limited sample. Standard isobologram analysis was performed to determine the extent and direction of the anti-leukemic interaction between the two agents. The IC₅₀ values of each drug are plotted on the axes; the solid line represents the additive effect, while the points represent the concentrations of each drug resulting in 50% inhibition of proliferation. Points falling below the line indicate synergistic effect, whereas those above the line indicate antagonistic effect.