Figure 3.

Regulation of ROS1 expression by miR-300 in LSCC. A. The Targetscan data showed that there were complementary seed region in the 3’-UTR of human ROS1 complementary to miR-300. B. miR-300 overexpression decreased the luciferase activity of WT 3’-UTR of ROS1 construct, however; overexpression of miR-300 did not change the luciferase activity of MUT 3’-UTR of ROS1 construct. C. The mRNA expression of ROS1 was measured by qRT-PCR. D. The protein expression of ROS1 was detected by western blot. ***p<0.001.