Skip to main content
. 2016 Sep 15;8(9):3976–3985.

Figure 3.

Figure 3

Effect of rapamycin and 3-MA treatment after spermine pretreatment on viability and apoptosis of immature cardiomyocytes exposed to hypoxia/ischemia. A. Cell viability was determined with CCK-8 assay in different conditions. *P<0.05 vs Control; #P<0.05 vs IRI. B. Representative immunofluorescent images of immature cardiomyocytes subjected to different conditions (left panels). Proliferating cells were stained with EDU, and total cells were stained with Hoechest 3344. Ratios of EDU-positive cells in total cells were calculated and analyzed (right panels). *P<0.05 vs Control; #P<0.05 vs IRI. C. Western blot was performed to evaluate Bcl-2, Bax expression in different conditions (upper panels). Quantization for Bax and Bcl-2 were normalized to ß-actin (bottom panels). *P<0.05 vs Control; #P<0.05 vs IRI.