Table 2.
Identification of immunoreactive proteins isolated from Bifidobacterium strain.
| Strain number | Estimated molecular weight of isolated protein [kDa] | Homologous protein name Accession No. | Nominal molecular weight of homologous proteins [kDa] | Origin of homologous protein | Homology [%] | Method of isolation | Immunoreactivity |
|---|---|---|---|---|---|---|---|
| Bifidobacterium longum ssp. longum CCM 7952 | 43 | Enolase ZP_00120494.2 | 46.6 | Bifidobacterium longum DJO10A | 38 | VI | Rabbit sera against B. longum ssp. longum CCM 7952 |
| 20 | Aspartokinase WP_007051616.1 | 18.6 | Bifidobacterium longum NCC2705 | 47 | V | Rabbit sera against B. longum ssp. longum CCDM 372 | |
| 55 | Pyruvate kinase ADC85999.1 | 52.5 | Bifidobacterium animalis subsp. lactis BB12 | 70 | IV, V | Rabbit sera against B. longum ssp. longum CCDM 372 | |
| 67 | Molecular chaperone DnaK NP_695712.1 | 66.9 | Bifidobacterium longum NCC2705 | 30 | III, IV, V | Rabbit sera against B. longum ssp. longum CCDM 372 | |
| 60 | Hypothetical protein BIFLAC_06461 ZP_02963269.1 | 62 | Bifidobacterium animalis subsp. lactis HN019 | 30 | III, VI | Rabbit sera against L. paracasei LCOK 0912 | |
| Bifidobacterium longum ssp. longum CCDM 372 | 40 | Sugar ABC transporter ATP-binding protein NP_695858.1 | 40.7 | Bifidobacterium longum NCC2705 | 37 | VI | Sera from germ mice colonized with Bifidobacterium longum ssp. longum CCDM 372 |
| 94 | Not identified | III, IV, V, VI | Rabbit sera against B. longum ssp. longum CCDM 372 | ||||
| 66 | Penicillin-binding protein 3 peptidoglycan synthetase EDT88982.1 | 65.3 | Bifidobacterium animalis subsp. lactis HN019 | 65 | III, IV, V | Rabbit sera against B. longum ssp. longum CCM 7952 or CCDM 372 | |
| 55 | Pyruvate kinase WP_014697813.1 | 52.6 | Bifidobacterium animalis subsp. animalis ATCC 25527 | 53 | III, IV, V | Rabbit sera against B. longum ssp. longum CCDM 372 | |
| 43 | Phosphoglycerate kinase NP_695890.1 | 46.6 | Bifidobacterium longum NCC2705 | 39 | III, IV, V, VI | Rabbit sera against B. longum ssp. longum CCM 7952 or CCDM 372 | |
| 35 | Transaldolase ZP_04664439.1 | 39.7 | Bifidobacterium longum subsp. infantis CCUG 52486 | 24 | III, V | Rabbit sera against B. longum ssp. longum CCDM 372 | |
| 30 | 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase ZP_02963768.1 | 25.7 | Bifidobacterium animalis subsp. lactis HN019 | 50 | VI | Rabbit sera against B. longum ssp. longum CCM 7952 or CCDM 372 | |
| 22.6 | 50S ribosomal protein L5 ZP_02963325.1 | 22 | Bifidobacterium animalis subsp. lactis HN019 | 40 | VI | Rabbit sera against B. longum ssp. longum CCM 7952 or CCDM 372 | |
| 20 | 30S ribosomal protein S9 BAJ69893.1 | 17.5 | Bifidobacterium longum subsp. infantis ATCC 15697 | 73 | III | Rabbit sera against B. longum ssp. longum CCM 7952 or CCDM 372 | |
| 17 | 30S ribosomal protein S16 Q8G7G1.1 | 16.4 | Bifidobacterium longum NCC 2705 | 79 | III | Rabbit sera against B. longum ssp. longum CCM 7952 or CCDM 372 | |
| 14 | 50S ribosomal protein L15 WP_008783622.1 | 15.8 | Bifidobacterium longum subsp. infantis ATCC 15697 | 60 | III | Rabbit sera against B. longum ssp. longum CCM 7952 or CCDM 372 | |
| 45 | Glyceraldehyde 3-phosphate dehydrogenase C KFI73119.1 | 39.3 | Bifidobacterium minimum | 40 | III | Human sera from patients with acute C. difficile infection | |
| 60 | Hypothetical protein BIFLAC_06461 ZP_02963269.1 | 62 | Bifidobacterium animalis subsp. lactis HN019 | 30 | III, V, VI | Rabbit sera against L. paracasei LOCK 0912 |