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. Author manuscript; available in PMC: 2016 Oct 4.
Published in final edited form as: Lipids. 2013 Nov 20;49(1):49–57. doi: 10.1007/s11745-013-3863-6

Figure 4. Effects of PDX on fMLF-induced p47phox phosphorylation on Ser345.

Figure 4

Human neutrophils (2 × 106 cells/mL HBSS) were pretreated with 5 or 10 μM PDX for 20 minutes, then incubated with or without (control) fMLF (0.1 μM) for 20 minutes. Cells were then lysed and proteins were analyzed with SDS-PAGE and immunoblotting with anti-phospho-ser345 antibody (pSer345) or anti-p47phox antibody (p47phox) (A). Western blots from different experiments were scanned, phosphorylated and total p47phox were quantified by densitometry, and the intensity of phosphorylated p47phox was corrected for the amount of p47phox (B). Data are representative of 3 independent experiments using cells from different healthy donors. Results are expressed as mean ± SD (n=3). Different letters indicate significant differences between the groups at p< 0.05 level. One away ANOVA followed by Fisher’s PLSD test.