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. 2016 Sep 28;1(5):e00250-16. doi: 10.1128/mSphere.00250-16

FIG 5  .

FIG 5 

Rom2 protein levels in the ccr4Δ, khd1Δ ccr4Δ, ccr4Δ mpk1Δ, and khd1Δ ccr4Δ mpk1Δ mutant strains. The Rom2myc protein levels in wild-type, ccr4Δ, khd1Δ ccr4Δ, ccr4Δ mpk1Δ, and ccr4Δ khd1Δ mpk1Δ cells are shown. Wild-type (180-m-1D4A), khd1Δ ccr4Δ (180-m-3D), khd1Δ ccr4Δ mpk1Δ (180-m-7A), ccr4Δ (180-m-6B), and ccr4Δ mpk1Δ (180-m-4C) cells harboring the ROM2myc construct were cultured to mid-logarithmic phase in YPD containing 10% sorbitol medium and collected, and total protein was prepared. The Rom2myc proteins were quantified by Western blotting as described in Materials and Methods. Pgk1 protein was included as a quantity control. The protein levels are indicated as percentages of wild-type levels and represent the means ± standard deviations from three independent experiments.