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. 2016 Mar 26;10:66–74. doi: 10.1016/j.btre.2016.03.006

Fig. 1.

Fig. 1

Induction of crocus calli from five selected Turkish crocus species (Crocus specious ssp. Specious, Crocus oliveri ssp. Oliveri, Crocus pestalozzae, Crocus abantensis, and Crocus paschei (C. abantensis × C. oliveri ssp. Oliveri) and plant regeneration from leaf, stem and corm explants (a-r). Callus induction on the bottom surface of leaf (a-e) as well as well-developed globular somatic embryos at different stages of development (arrow heads) from leaf explants within three months of culture on MS medium supplemented with 4 mg/L α-naphthaleneacetic acid (NAA) and 4 mg/L thidiazuron (TDZ) (b, e); stem (f-i) and corm (k-o) explants after three months of culture on MS medium with 4 mg/L α-naphthaleneacetic acid (NAA) and 4 mg/L thidiazuron (TDZ). A plantlet regenerated from well-developed callus after six months of culture on MS medium supplemented with 2 mg/L indole-3-acetic acid (IAA) plus 2 mg/L thidiazuron (TDZ) and 6-benzylaminopurine (BAP) (o-r).