Fig. 3.

Plant regeneration through indirect somatic embryogenesis from the leaf explants of crocus species Crocus oliveri ssp. Oliveri. Well-developed globular somatic embryos (a); developing stages of cotyledonary stages of somatic embryos (b-d); on the callus from leaf explants after three months of culture on MS medium supplemented with 2 mg/L indole-3-acetic acid (IAA) plus 2 mg/L thidiazuron (TDZ) and 100 mg/L abscisic acid (ABA). (e) Well-developed cotyledonary stage somatic embryos. (f) A plantlet regenerated from a somatic embryo. (h): Regenerated plants in the potted soil.