Table 2.
Development of cotyledonary somatic embryos from the different explants (leaf, stem and corm) of the five crocus species cultured in the presence of the four different media. Each medium is supplemented with (2 mg/L NAA + 2 mg/L TDZ) and 100 mg/L ABA. Data were collected after five weeks of culturing.
| Crocus species | Explants | Callus response for cotyledonary somatic embryos |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| MS medium |
GB5 medium |
LS medium |
CHE’ medium |
||||||
| Average number of embryos formeda | Percent frequency of embryos formed |
Average number of embryos formeda | Percent frequency of embryos formed | Average number of embryos formeda | Percent frequency of embryos formed | Average number of embryos formeda | Percent frequency of embryos formed | ||
| Crocus specious ssp. Specious | Leaf | 2.5 ± 0.2b | 36.6b | 1.2 ± 0.4b | 13.3c | 1.4 ± 0.3c | 36.6a | 1.3 ± 0.6dc | 43.3a |
| Stem | 1.1 ± 0.4d | 23.3e | 1.0 ± 0.3c | 10.0d | 1.5 ± 0.7c | 10.0e | 1.0 ± 0.4de | 16.6g | |
| Corm | 1.5 ± 0.2c | 26.6d | 1.0 ± 0.3c | 13.3c | 1.3 ± 0.6c | 16.6d | 1.1 ± 0.4dc | 30.0d | |
| Crocus oliveri ssp. Oliveri | Leaf | 2.9 ± 0.4a | 46.6a | 1.5 ± 0.2a | 16.6b | 1.3 ± 0.6c | 33.3b | 1.8 ±0.4a | 40.0b |
| Stem | 1.5 ± 0.2c | 30.0c | 1.1 ± 0.1cb | 20.0a | 1.8 ± 0.4b | 16.6d | 1.4 ± 0.3bc | 23.3e | |
| Corm | 1.8 ± 0.4c | 36.6b | 1.1 ± 0.1cb | 16.6b | 2.0 ± 0.3a | 26.6c | 1.7 ± 0.7ab | 33.3c | |
| Crocus pestalozzae | Leaf | 2.0 ± 0.3b | 26.6d | 0.6 ± 0.3d | 13.3c | 0.7 ± 0.3d | 16.6d | 1.0 ± 0.3dc | 23.3e |
| Stem | 0.7 ± 0.3d | 6.6g | 0.2 ± 0.3e | 3.3f | 0.2 ± 0.3e | 3.3g | 0.4 ± 0.2e | 10.0h | |
| Corm | 0.9 ± 0.6d | 13.3f | 0.3 ± 0.3e | 6.6e | 0.4 ± 0.2e | 6.6f | 0.6 ± 0.3e | 20.0f | |
| Crocus abantensis | Leaf | – | – | – | – | – | – | – | – |
| Stem | – | – | – | – | – | – | – | – | |
| Corm | – | – | – | – | – | – | – | – | |
|
Crocus paschei (C. abantensis × C. oliveri ssp. Oliveri) |
Leaf | – | – | – | – | – | – | – | – |
| Stem | – | – | – | – | – | – | – | – | |
| Corm | – | – | – | – | – | – | – | – | |
a
Mean number of somatic embryos formed per callus explant ± SE (standard error) with the same letter within columns are not significantly different according to Duncan’s multiple range test at p < 0.05.