Table 1.
Combinatorial protein microarrays layout used for array synthesis. The following 11 proteins were used: Type I collagen (Col I), type II collagen (Col II), type III collagen (Col III), type IV collagen (Col IV), laminin (Ln), fibronectin (Fn), fibroblast growth factor 2 (FGF-2), insulin-like growth factor 2 (IGF-2), vascular endothelial growth factor (VEGF), exenatide (Exen) and vitronectin (Vn). For single proteins and binary combinations, Col I, Col II, Col III, Col IV, Ln and Fn were printed at a concentration of 100 µg/mL; FGF-2, IGF-2, VEGF and Exen at a concentration of 25 µg/mL; and Vn at 50 µg/mL. In ternary combinations, the first protein listed was printed as the major component, at the concentration listed above, the second and third listed components were printed at 2/3 the concentration of the first listed protein.
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A | Col I | Col I/ Ln |
Col II/ Exen |
Col IV/ VEGF |
FGF-2/ GF-2 |
Col IV/ Col I/ FGF-2 |
Col IV/ Col III/ Fn |
Col IV/ Fn/ VEGF |
Ln/ Col I/ Col III |
Ln/ Col II/ Exen |
Ln/ Col IV/ Vn |
Ln/ VEGF/ Vn |
FGF-2/ Col II/ Fn |
FGF-2/ Col IV/ IGF-2 |
Col IV/ IGF-2/ Exen |
| B | Col II | Col I/ Fn |
Col II/ /Vn |
Col IV/ Exen |
FGF-2 / VEGF |
Col IV/ Col I/ IGF-2 |
Col IV/ Col III/ FGF-2 |
Col IV/ Fn/ Exen |
Ln/ Col I/ Col IV |
Ln/ Col II/ Vn |
Ln/ Fn/ FGF-2 |
Ln/ Exen/ Vn |
FGF-2/ Col II/ IGF-2 |
FGF-2/ Col IV/ VEGF |
Col IV/ IGF-2/ Vn |
| C | Col III | Col I/ FGF-2 |
Col III/ Col IV |
Col IV/ Vn |
FGF-2/ Exen |
Col IV/ Col I/ VEGF |
Col IV/ Col III/ IGF-2 |
Col IV/ Fn/ Vn |
Ln/ Col I / Fn |
Ln/ Col III/ Col IV |
Ln/ Fn / IGF-2 |
FGF-2/ Col I/ Col II |
FGF-2/ Col II/ VEGF |
FGF-2/ Col IV/ Exen |
Col IV/ VEGF/ Exen |
| D | Col IV | Col I/ IGF-2 |
Col III/ Ln |
Ln/ Fn |
FGF-2/ Vn |
Col IV/ Col I/ Exen |
Col IV/ Col III/ VEGF |
Col IV/ FGF-2/ IGF-2 |
Ln/ Col I/ FGF-2 |
Ln/ Col III/ Fn |
Ln/ Fn VEGF |
FGF-2/ Col I/ Col III |
FGF-2/ Col II/ Exen |
FGF-2/ Col IV/ Vn |
Col IV/ VEGF/ Vn |
| E | Ln | Col I/ VEGF |
Col III/ Fn |
Ln/ FGF-2 |
IGF-2/ VEGF |
Col IV/ Col I/ Vn |
Col IV/ Col III/ Exen |
Col IV/ FGF-2/ VEGF |
Ln/ Col I / IGF-2 |
Ln/ Col III/ FGF-2 |
Ln/ Fn/ Exen |
FGF-2/ Col I/ Col IV |
FGF-2/ Col II/ Vn |
FGF-2/ L / Fn |
Col IV/ Exen/ Vn |
| F | Fn | Col I/ Exen |
Col III/ FGF-2 |
Ln/ IGF-2 |
IGF-2/ Exen |
Col IV/ Col II/ Col III |
Col IV/ Col III/ Vn |
Col IV/ FGF-2/ Exen |
Ln/ Col I/ VEGF |
Ln/ Col III/ IGF-2 |
Ln/ Fn/ Vn |
FGF-2/ Col I/ Ln |
FGF-2/ Col III/ Col IV |
FGF-2/ Ln/ IGF-2 |
|
| G | FGF-2 | Col I/ Vn |
Col III/ IGF-2 |
Ln/ VEGF |
IGF-2/ Vn |
Col IV/ Col II/ Ln |
Col IV/ Ln/ Fn |
Col IV/ FGF-2/ Vn |
Ln/ Col I/ Exen |
Ln/ Col III/ VEGF |
Ln/ FGF-2/ IGF-2 |
FGF-2/ Col I/ Fn |
FGF-2/ Col III/ Ln |
FGF-2/ Ln/ VEGF |
|
| H | IGF-2 | Col II/ Col III |
Col III/ VEGF |
Ln/ Exen |
VEGF/ Exen |
Col IV/ Col II/ Fn |
Col IV/ Ln/ FGF-2 |
Col I/ IGF-2/ VEGF |
Ln/ Col I/ Vn |
Ln/ Col III/ Exen |
Ln/ FGF-2/ VEGF |
FGF-2/ Col I/ IGF-2 |
FGF-2/ Col III/ Fn |
FGF-2/ Ln/ Exen |
|
| I | VEGF | Col II/ Col IV |
Col III/ Exen |
Ln/ Vn |
VEGF/ Vn |
Col IV/ Col II/ FGF-2 |
Col IV/ Ln/ IGF-2 |
Col I/ IGF-2/ Exen |
Ln/ Col II/ Col III |
Ln/ Col III/ Vn |
Ln/ FGF-2/ Exen |
FGF-2/ Col I/ VEGF |
FGF-2/ Col III/ IGF-2 |
FGF-2/ Ln / Vn |
|
| J | Exen | Col II/ Ln |
Col III/ Vn |
Fn/ FGF-2 |
Exen/ Vn |
Col IV/ Col II/ IGF-2 |
Col IV/ Ln/ VEGF |
Col I/ IGF-2/ Vn |
Ln/ Col II/ Col IV |
Ln/ Col IV/ Fn |
Ln/ FGF-2/ Vn |
FGF-2/ Col I/ Exen |
FGF-2/ Col III/ VEGF |
FGF-2/ Fn/ IGF-2 |
|
| K | Vn | Col II/ Fn |
Col IV/ Ln |
Fn/ IGF-2 |
Col IV/ Col I Col II |
Col IV/ Col II/ VEGF |
Col IV/ Ln/ Exen |
Col IV/ EGF/ Exen |
Ln/ Col II/ Fn |
Ln/ Col IV/ FGF-2 |
Ln/ IGF-2/ VEGF |
FGF-2/ Col I/ Vn |
FGF-2/ Col III/ Exen |
FGF-2/ Fn VEGF |
|
| L | Col I/ Col II |
Col II/ FGF-2 |
Col IV/ Fn |
Fn/ VEGF |
Col IV/ Col I/ Col III |
Col IV/ Col II/ Exen |
Col IV/ Ln/ Vn |
Col I/ VEGF/ Vn |
Ln/ Col II/ FGF-2 |
Ln// Col IV/ IGF-2 |
Ln/ IGF-2/ Exen |
FGF-2/ Col II/ Col III |
FGF-2/ Col III/ Vn |
FGF-2/ Fn/ Exen |
|
| M | Col I/ Col III |
Col II/ IGF-2 |
Col IV/ FGF-2 |
Fn/ Exen |
Col IV/ Col I/ Ln |
Col IV/ Col II/ Vn |
Col IV/ Fn/ FGF-2 |
Col I/ Exen/ Vn |
Ln/ Col II / IGF-2 |
Ln/ Col IV/ VEGF |
Ln/ IGF-2/ Vn |
FGF-2/ Col II/ Col IV |
FGF-2/ Col IV/ Ln |
FGF-2/ Fn/ Vn |
|
| N | Col I/ Col IV |
Col II/ VEGF |
Col I/ IGF-2 |
Fn/ Vn |
Col IV/ Col I/ Fn |
Col IV/ Col III/ Ln |
Col IV/ Fn/ IGF-2 |
Ln/ Col I/ Col II |
Ln/ Col II/ VEGF |
Ln/ Col IV/ Exen |
Ln/ VEGF/ Exen |
FGF-2/ Col II/ Ln |
FGF-2/ Col IV Fn |
Col IV/ IGF-2/ VEGF |