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. 2016 Aug 24;8(9):77. doi: 10.3390/cancers8090077

Figure 3.

Figure 3

Effects of HSP90 inhibitors upon CAF contractility and mobility. (A) Radicicol inhibited collagen gel contraction (CGC) by CAF in a dose-dependent manner (p < 0.001 for all doses and time points vs. vehicle control; n = 21 experiments in triplicates for 0 nM, n = 18 for 100 nM and n = 16 for 300 nM with a total of 11 different CAF lines; unpaired two-sided t-test); (B) Treatment with radicicol did not reduce cell viability, as measured with an MTS assay after the CGC (n = 10 independent experiments and CAF lines; repeated measures ANOVA, p > 0.05; not significant). NT = no treatment; 0 nM received the dose of vehicle comparable to the highest treatment dose; (C) 17-DMAG significantly inhibited collagen gel contraction in a dose-dependent manner (n = 3 CAF lines and independent experiments in triplicates; repeated measures ANOVA, p < 0.001 for all doses); (D) 17-DMAG showed little effect upon cell viability in an MTS assay of CAF after the CGC except for the highest dose of 60 nM (n = 3 independent experiments and CAF lines; repeated measures ANOVA, p < 0.01); (E) Radicicol treatment (300 nM) reduced CAF migration in wound closure (WC) in the scratch assay after 24 h. n = 5 CAF populations, paired, two-sided t-test. NT = no treatment; (F) 30 nM 17-DMAG reduced migration in the scratch assay in CAF after 24 h. n = 5 CAF populations, p < 0.01 paired, two-sided t-test. All panels: mean + S.E.M. and asterisks denote the level of significance: * for p < 0.05, ** for p < 0.01 and *** for p < 0.001. Incubation times: CGC 48 h, MTS 3 h, WC 24 h.