Skip to main content
. 2016 Aug 24;8(9):77. doi: 10.3390/cancers8090077

Figure 5.

Figure 5

Contractility but not motility regulated via the ROCK pathway. Effects of ROCK inhibitors upon CAF collagen contractility was measured, after which the cell viability was examined by MTS assay. (A) The ROCK inhibitor Y-27632 impaired CAF contractility and showed no cytotoxic effects in an MTS assay (B); (C) The ROCK inhibitor fasudil inhibited CAF contractility with little cytotoxicity (D); In the scratch assay, Y-27632 (E) and fasudil (F) had no effect. (A–D) CAF from n = 3 different patients; repeated measures ANOVA; (E,F) CAF from n = 4 patients; paired, two-sided t-test. All panels: mean + S.E.M. and asterisks denote the level of significance * for p < 0.05, ** for p < 0.01 and *** for p < 0.001. n.s. = not significant. Incubation times: CGC 48 h, MTS 3 h, WC 24 h.