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. 2016 Aug 2;4(3):27. doi: 10.3390/vaccines4030027

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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HCV peptide-specific T-cell lines grown from PBMC frozen pre-vaccination in HCV infected patients: (A) PBMC frozen pre-vaccination from HLA-A2+ patients who showed a positive response to NS3₁₄₀₆ after vaccination were cultured with the 15mer (LAAKLSGLGINAVAY; optimal epitope highlighted in bold) peptide and recombinant human IL-2 for 19 days. PBMC were stained with MHC class I pentamer (NS3₁₄₀₆ KLSGLGINAV) at day 0, 12, and 19 of the culture; and (B) example plots for the two patients for which NS3₁₄₀₆-specific T-cells were grown to detectable levels at day 19 from pre-vaccine PBMC (patients 351, 357. Negative at day 19: patients 355 and 360).