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The anthrax biochip built from the sequential deposition of (a) a tethered bilayer lipid membrane, and (b) self-assembling B. anthracis PA63 ion channels (nanopores) [41]—the selective sensing element; (c) B. anthracis Lethal Factor [53] is detected by binding to the PA63 channel cap domain [43] via electrochemical impedance spectroscopy and surface plasmon resonance.
