Figure 5. NS8593 blocks [Ca²⁺]_i and Sr²⁺ induced oscillations in oocytes.

Spontaneous [Ca²⁺]_i oscillations were induced and measured at RT in response to increasing concentrations of extracellular Ca²⁺ and 10 mM Sr²⁺ in WT oocytes. (a) Left panel, [Ca²⁺]_i oscillations were evaluated in response to increased extracellular Ca²⁺ (black horizontal bars). Figure shows representative traces for 3 oocytes. n = 9. Right panel: Oocytes were exposed to 10 μM NS8593 (cyan bar) and Ca²⁺ oscillations were evaluated after increasing external Ca²⁺ concentration from 2 or 5 mM (black horizontal bars). n = 13. (b) Left panel: Representative traces for 3 oocytes showing Sr²⁺-induced oscillations. n = 5, oocytes that show 3 or more oscillations. Right panel: Sr²⁺-induced oscillations were blocked by exposure to 10 μM NS8593 (cyan bar). n = 9. (c) Oscillations induced by Sr²⁺ at 33–35 °C (left panel, n = 5. Figure shows representative traces for 3 eggs) are not blocked by 10 μM NS8593 (cyan bar; right panel, n = 7. Figure shows representative traces for 3 eggs).