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. 2016 Sep 29;6:34238. doi: 10.1038/srep34238

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) The expression of miR-24 in the serum of 62 OS patients and 62 healthy controls was detected by using qRT-PCR analysis. *p < 0.05. The means of RNA expression were determined by using the 2^−△△CT analysis method, and normalized to U6 snRNA. The average miR-24 level in the serum of all healthy controls was normalized to the average serum level of all OS patients and a p < 0.05 was considered significant. (B) qRT-PCR analysis of miR-24 expression was conducted on 45 pairs of primary OS tissue samples and adjacent non-tumor tissue samples as well as four OS cell lines. *p < 0.05 vs. non-tumor tissues group. The average miR-24 levels of all the four OS cell lines and all OS tumor tissues were normalized to the average level of all non-tumor tissues and a p < 0.05 was considered significant. All data represent the mean ± SD from at least three independent experiments in each condition.