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. 2016 Sep 29;6:33980. doi: 10.1038/srep33980

Figure 4. The expression of endogenous growth factors by primary hepatocytes is affected by small volumes of microchambers.

Figure 4

(a) Quantitative RT-PCR analysis of important liver-relate growth factors at day 7. Fold increase is relative to values obtained for hepatocytes cultured in standard tissue culture. (b) Quantitative RT-PCR analysis of HGF expression over 9 days of hepatocyte culture inside μCs and in 12-well plates. (c) HGF ELISA of hepatocyte cultured for 7 days μCs and in standard culture plates. (d) Quantitative RT-PCR analysis of TGF-β1 inside μCs and in 12-well plates. Data represent the average of 3 biological samples ± SD. *p < 0.05.