Skip to main content
. 2016 Sep 29;6:33146. doi: 10.1038/srep33146

Figure 2. Effect of BA145 on apoptosis induction in PANC-1 cells.

Figure 2

BA145 did not induces apoptosis in PANC-1 cells as determined by ApoBrdU assay, DNA fragmentation and western blotting (a) Cells were treated with different concentrations of BA145 for 24 h and then stained with ApoBrdU DNA fragmentation kit (APO-DIRECT™ Kit). The apoptotic BrdU positive cells were examined by flow cytometry (b) DNA fragmentation assay. Genomic DNA was extracted from BA145 treated cells at indicated doses for 24 h and DNA fragmentation was examined by agarose gel electrophoresis (c) Effect of BA145 on the cleavage of procaspase-3 and PARP-1 in PANC-1. Cells were treated with BA145 at indicated doses and time intervals, whole cell lysates were prepared and proteins are resolved on SDS gel for western blot analysis. β-actin was used as a loading control.