Skip to main content
. 2016 Sep 29;6:33146. doi: 10.1038/srep33146

Figure 4. Role of autophagy in BA145 mediated G2/M arrest.

Figure 4

(a) Pharmacological inhibition of autophagy arbitrated BA145 mediated G2/M arrest in PANC-1 cells. PANC-1 cells were co-treated with BA145 (40 μM) and/or the autophagy inhibitors bafilomycin (10 nM) and CQ (10 μM) for 12 h. Autophagy inhibitors were added 1 h before BA145 treatment. Cells were stained with PI and analyzed for cell cycle phase distribution by flow cytometry (b) Effect of autophagy inhibitors on the expression of G2/M regulatory proteins in BA145 treated PANC-1 cells. Cells were treated with BA145 and/or autophagy inhibitors as above described concentrations for 12 h and expression of various proteins were accessed by western blotting (c) Influence of beclin inhibition on the expression of G2/M regulatory proteins in BA145 (40 μM) treated PANC-1 cells. After BA145 treatment for 12 h, whole cell protein lysates were prepared for western blotting and the detection of the indicated proteins. β-actin was used as a loading control (d) Influence of Beclin inhibition on cell cycle phase distribution in BA145 (40 μM) treated PANC-1 cells for 12 h.