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. 2016 Sep 29;6:34529. doi: 10.1038/srep34529

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Copyright © 2016, The Author(s)

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(A) Schematic presentation of PCGEM1 promoter, and the location of the respective PCR primers for pulldown assays. The numbers above the PCGEM1 indicate the position relative to TSS. (B) A representative silver-stained gel picture showing a band unique to a biotin-labeled probe 5.2/3.1. Mass spectrometry analysis indicated that this band is Non-POU domain-containing octamer-binding protein (NONO), also known as p54/nrb. (C) Verification of the interaction of PCGEM1 with p54/nrb by p54/nrb antibody for the same precipitates as in (B). (D) The interaction of p54/nrb with PCGEM1 promoter by ChIP assays using primers PCGEM1-ChIP-5.1 and PCGEM1-ChIP-3.1 in 22Rv1 cells. Full-length gels and blots are included in the Supplementary Information file.