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. 2016 Sep 29;6:34529. doi: 10.1038/srep34529

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Two independent CWR22Rv1 p54/nrb knockout (KO) clones #11 and #24 were used for rescue experiments. Re-expression of p54/nrb significantly increases the PCGEM1 level. (B) Re-expression of p54/nrb significantly increases the AR3 level, as determined by Western blot. (C) Ectopic expression of PCGEM1 in p54/nrb KO #11 cells increases AR3 as detected by qRT-PCR. The exogenous p54/nrb was tagged with Myc. (D) Re-expression of p54/nrb enhances tumor cell growth. KO #11 and #24 cells were transduced with pCDH-Myc vector (vector) or pCDH-Myc-p54/nrb (p54/nrb) and then plated out in 96-well plates in the absence of androgen. Relative cell growth was measured by MTT assays at Day 6. Values are mean ± SE (n = 3). **p < 0.01; *p < 0.05. Full-length gels and blots are included in the Supplementary Information file.