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. 2016 Sep 29;6:33975. doi: 10.1038/srep33975

Figure 5. Increased Homer1a protein levels attenuated TG-induced SCOE-mediated calcium entry.

Figure 5

After infection with a lentivirus-mediated vector for either Homer1a shRNA or Homer1a O/E for 48 h, HT-22 cells were loaded with Fura-red AM (4 μM) and baseline levels were recorded every 15 s for a total of 100 s. The HT-22 cells were then treated with 2.5 μM TG to release Ca2+ from calcium stores (gray zone) in Ca2+-free buffer (black dotted line) for 500 s. The buffer was then refreshed with Ca2+ buffering (2 mM, black full line). Changes in Ca2+ signals were evaluated as time-series curve (A) and assessed as peak F/F0 (B) and area under curve (AUC, C). Approximately 40 to 50 cells were analyzed in each experiment. Data are presented as mean ± SEM from four experiments. *p < 0.05 vs. control cells.