Figure 2.

Multivariate modeling of DMEM (Dulbecco’s Modified Eagle Medium) media metabolic profiles: (A) PCA scores plot demonstrate a temporal shift in the cellular metabolic profile from 18 h to 22 h as note by the arrows; (B) (Orthogonal Partial Least Square-Discriminant Analysis) OPLS-DA scores plot showing distinct clustering between the pre- and post-shift time points; (C) OPLS modeling using time as the regressor variable led to a robust predictive model as suggested from the strong linearity between actual time points and those predicted from the model; and (D) heat map and hierarchical cluster of the metabolites significantly related to time points from supervised multivariate modeling (Both OPLS-DA and OPLS).