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. 2016 Sep 29;6:34324. doi: 10.1038/srep34324

Figure 2. Knockdown of SIRT1 promotes neurogenesis in P19 cells.

Figure 2

(a) (Upper panel) SIRT1 knockdown P19 cells were cultured for 9 days without any treatment. (Lower panel) P19 cells were treated with 100 μM EX-527 for 9 days. The SIRT1 knockdown cells and EX-527 treated cells were immunostained with antibodies against neuron-specific proteins (scale bar; 50 μm). (b) The expression levels of neuron-specific markers in SIRT1 knockdown P19 cells were determined at various times by using western blot and (c) RT-PCR analyses.