Figure 9. MCs were serum-starved for 24 h and pretreated with 17-phenyl trinor PGE2 (1.0 μM), butaprost (1.0 μM), sulprostone (1.0 μM) and cay10580 (1.0 μM) for 30 min before stimulation with TGF-β1 (10 ng/ml) for 10 min.
Total protein extracts were analysed by Western blotting using specific antibodies for total and phosphorylated ERK1/2, p38 MAPK, phosphorylated ERK1/2, p38 MAPK protein expression levels were analysed by Western blotting. Data are expressed as the S.E.M. (*P<0.05 compared with Control group, #P<0.05 compared with 10 ng/ml TGF-β1 group).